Chambers & Sliders

UssingChamber.com compares the different chambers & sliders that are currently available on the market:

 

Components
Warner Instruments
Physiologic Instruments
World Precision Instruments
Chamber material
Acrylic plastic
Acrylic plastic
Acrylic plastic
Interchangeable Sliders
Yes - Chamber splits for sliders to be insterted from the top.
Yes - Chamber splits for the sliders to be inserted from the side.
No Sliders -Chamber splits for material
Leak Liquid?
Yes - Lots of leakage out of sides and bottom.
No - Once slider is set no leaks
Yes - Lots of leakage from bottom when separating the chamber system.
Ease of Use
No easy way to pull sliders without separating chambers. Chambers have no quick release system.
Quite easy to pull out slider with quick release system. Minimal liquid in chamber.
Simple chamber system - Requires quite a bit of space.  Once chamber is separated you have lots of liquid that releases from the exchange above. Very messy.
Tissue Pressure
Pressure is minimal because of small cube design.
Pressure is minimal because of small cube design.
Pressure on tissue due to design of the exchange above the chamber. Can be harmful to your tissue.

Tell Us Your Experience With The Chambers

More on our choice of chambers to use::

Physiologic Instruments Inc. - RANKING #1 Best For Chambers

Physiologic Instruments (San Diego, CA USA)- Manufactors a Ussing Chamber that is made from acrylic plastic that are machined to the specifications of the experiment. Physiologic Instruments has three different types of chambers; P2250, P2300, & P2400 (reference information from www.physiologicinstruments.com).

P2250 Ussing Chamber

The P2250 ussing chambers specifically designed for mounting intestinal tissues using an oblong aperture 5 x 22 mm to yield 1.0 cm2 tissue area. Tissues are mounted by pressing the tissue over short, curved pins lining a matching aperture on a slider (P/N P2252).Usable half chamber volume range is 2.0 - 8.0 ml.

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P2300 Ussing Chamber

The P2300 ussing chamber is our most versatile chamber in that it also directly accommodates sliders (holders) for a variety of animal tissues. Usable halfchamber volume is 2.0 - 8.0 ml.

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P2400 Ussing Chamber

The P2400 is a low volume ussing chamber system for used to study transport in tissues where the net quantity transported is low or the assay for the transported solute requires higher concentrations. This system, like the system for Snapwell culture inserts above, permits a variety of tissue sizes to be mounted in a low volume chamber.

 

Warner Instruments -

The U9926 and U2500 Self-Contained Ussing chambers improve on the Classic design by bringing the solution reservoir into the chamber housing. This dramatically reduces the size of the apparatus and increases the ease-of-use of the system (reference information from www.warneronline.com).

The Self-Contained Ussing Chambers systems come in single- and dual-channel models and offer many advantages over the Classic design. The single-channel U9926 and dual-channel U2500. Both systems make use of inserts to secure and position the tissue or culture cup within the chamber body.

 

World Percision Instruments -

WPI's ussing chambers are machined from solid acrylic with eight entry ports for fluid lines, electrodes, or agar bridges. For easy, leak-free attachment of tubing and electrodes, all eight ports are luer type. The four ports for voltage and current electrodes are recessed to prevent formation of air bubbles in the chamber. The fluid compartments in each side of the chamber are separated by the epithelial membrane being studied. Sharp stainless steel pins on one side of the chamber hold the membrane in position and mate with holes in the opposite chamber interface (reference information from www.wpiinc.com).

 

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